Though we have not examined PneumaCult™ media to be used with murine epithelial cells, you will discover publications that report this application.
RNPs are sent to CD34+ cells by means of the CellPore™ Transfection System. Publish-transfection, cells are returned to society and subsequently analyzed for modifying efficiency by means of established strategies as permitted via the experimental design and style, for instance flow cytometry or ArciTect™ T7 Endonuclease I Kit (Catalog #76022). Edited progenitor cell purpose is usually calculated by in vitro colony-forming unit (CFU) assays or cells could be even more cultured for lineage-precise differentiation using tailor-made StemSpan™ media and dietary supplements.
If applying Gentle Cell Dissociation Reagent (GCDR), the period of GCDR remedy will have to be optimized. Incubate organoids with GCDR for eight -ten minutes at 37°C and make use of a P1000 pipette to Carefully pipette up and down, and visually inspect if organoids split up.
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See info showing superior ALI tradition morphology and better epithelial cell marker expression making use of these encouraged Transwell® inserts >
The readings can also be done prior to the tradition is evaluated for endpoint characterizations, like electrophysiology or airway marker expression.
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Here's a listing of revealed literature that cites culturing of nasal epithelial cells using PneumaCult™ tradition media:
Notes: STEMdiff™ Kidney Organoid Kit consists of a different plate format/culture set-up and is not a standard monolayer culture; kidney organoids protrude through the fundamental monolayer culture.
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